Session S58b (Wednesday, 2pm, Wartik 108)

P762: Lab exercise in DNA oxidation and protection by antioxidants

Laura Frost (Georgia Southern University, USA), Julia Brumaghim (Clemson University, USA)

A common biochemistry laboratory activity includes conducting a DNA electrophoresis experiment.  In the described exercise student teams explore oxidative damage to DNA through use of a photo-induced nicking agent visualized through electrophoresis.  This experiment is an adaptation of an exercise published by Shafirovich (J. Chem. Educ., 2003, 80, 1297-1299) with a new twist.  Polyphenolic antioxidant compounds commonly found in food can protect DNA from oxidative damage and the use of these is explored through the experiment.  Upon completion, teams orally present the results of their experiment to the class followed by a mandatory question and answer period. The merits and assessment of this activity will be presented.


A sample text widget

Etiam pulvinar consectetur dolor sed malesuada. Ut convallis euismod dolor nec pretium. Nunc ut tristique massa.

Nam sodales mi vitae dolor ullamcorper et vulputate enim accumsan. Morbi orci magna, tincidunt vitae molestie nec, molestie at mi. Nulla nulla lorem, suscipit in posuere in, interdum non magna.